Your browser is unsupported

We recommend using the latest version of IE11, Edge, Chrome, Firefox or Safari.

In this section
Photo of Hyun Lee

Hyun Lee

Director of Biophysics Core, Research Resources Center

Research Assistant Professor, Medicinal Chemistry and Pharmacognosy

, Center for Biomolecular Sciences

Contact Information

1100 S. Ashland Avenue
CfSB Rm 119
Chicago, IL 60607

Phone: (312) 355-5292


  • PhD, Biophysics, The Ohio State University, Columbus, Ohio, 2006
  • MS, Chemistry, Chonnam National University, Kwangju, South Korea, 1998
  • BS, Agricultural Biology, Chonnam National University, Kwangju, South Korea, 1995

Intellectual Contributions

Journal Article

  • Lee H, Ren J, Pesavento R, Ojeda I, Rice AJ, Lv H, Kwon Y, Johnson ME. "Identification and design of novel small molecule inhibitors against MERS-CoV papain-like protease via high-throughput screening and molecular modeling.". Bioorganic & medicinal chemistry. 2019;. doi:10.1016/j.bmc.2019.03.050.
  • Choules MP, Wolf N, Lee H, Anderson JR, Grzelak E, Wang Y, Ma R, Gao W, McAlpine JB, Jin YY, Cheng J, Lee H, Suh JW, Duc NM, Paik S, Choe JH, Jo EK, Chang CL, Lee J, Jaki BU, Pauli GF, Franzblau SG, Cho S. "Rufomycin Targets ClpC1 Proteolysis in Mycobacterium tuberculosis and M. abscessus.". Antimicrobial agents and chemotherapy. 2019;63(3). doi:10.1128/AAC.02204-18.
  • Koh KH, Cao Y, Mangos S, Tardi NJ, Dande RR, Lee HW, Samelko B, Altintas MM, Schmitz VP, Lee H, Mukherjee K, Peev V, Cimbaluk DJ, Reiser J, Hahm E. "Non-immune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist.". The Journal of clinical investigation. 2019;. doi:10.1172/JCI123386.
  • Oguri T, Kwon Y, Woo JKK , Prehna G, Lee H, Ning M, Won K, Lee J, Mei S, Shi Y, Jeong H, Lee H. "A family of small intrinsically disordered proteins involved in flagella-dependent motility in Salmonella.". Journal of bacteriology. 2018;. doi:10.1128/JB.00415-18.
  • Ren J, Dsouza NR, Deng H, Lee H, Bouvier M, Johnson ME. "Discovery of small molecule inhibitors of adenovirus by disrupting E3-19K/HLA-A2 interactions.". Bioorganic & medicinal chemistry letters. 2018;. doi:10.1016/j.bmcl.2018.07.036.
  • Moon HG, Kim SJ, Jeong JJ, Han SS, Jarjour NN, Lee H, Abboud-Werner SL, Chung S, Choi HS, Natarajan V, Ackerman SJ, Christman JW, Park GY. "Airway Epithelial Cell-Derived Colony Stimulating Factor-1 Promotes Allergen Sensitization.". Immunity. 2018;. doi:10.1016/j.immuni.2018.06.009.
  • Hevener KE, Santarsiero BD, Lee H, Jones JA, Boci T, Johnson ME, Mehboob S. "Structural characterization of Porphyromonas gingivalis enoyl-ACP reductase II (FabK).". Acta crystallographica. Section F, Structural biology communications. 2018;74(Pt 2):105-112. doi:10.1107/S2053230X18000262.
  • Hevener KE, Pesavento R, Ren J, Lee H, Ratia KM, Johnson ME. "Hit-to-Lead: Hit Validation and Assessment.". Methods in enzymology. 2018;610:265-309. doi:10.1016/bs.mie.2018.09.022.
  • Holman KM, Puppala AK, Lee J, Lee H, Simonovic M. "Insights into substrate promiscuity of human seryl-tRNA synthetase.". RNA (New York, N.Y.). 2017;. doi:10.1261/rna.061069.117.
  • Hayek SS, Koh KH, Grams ME, Wei C, Ko YA, Li J, Samelko B, Lee H, Dande RR, Lee HW, Hahm E, Peev V, Tracy M, Tardi NJ, Gupta V, Altintas MM, Garborcauskas G, Stojanovic N, Winkler CA, Lipkowitz MS, Tin A, Inker LA, Levey AS, Zeier M, Freedman BI, Kopp JB, Skorecki K, Coresh J, Quyyumi AA, Sever S, Reiser J. "A tripartite complex of suPAR, APOL1 risk variants and αvβ3 integrin on podocytes mediates chronic kidney disease.". Nature medicine. 2017;. doi:10.1038/nm.4362.
  • Lee H, Ren J, Nocadello S, Rice AJ, Ojeda I, Light S, Minasov G, Vargas J, Nagarathnam D, Anderson WF, Johnson ME. "Identification of novel small molecule inhibitors against NS2B/NS3 serine protease from Zika virus.". Antiviral research. 2016;. doi:10.1016/j.antiviral.2016.12.016.
  • Rice AJ, Lei H, Santarsiero BD, Lee H, Johnson ME. "Ca-asp bound X-ray structure and inhibition of Bacillus anthracis dihydroorotase (DHOase).". Bioorganic & medicinal chemistry. 2016;. doi:10.1016/j.bmc.2016.07.055.
  • Han KY, Chang JH, Lee H, Azar DT. "Proangiogenic Interactions of Vascular Endothelial MMP14 With VEGF Receptor 1 in VEGFA-Mediated Corneal Angiogenesis.". Investigative ophthalmology & visual science. 2016;57(7):3313-22. doi:10.1167/iovs.16-19420.
  • Lei H, Jones C, Zhu T, Patel K, Wolf NM, Fung LW, Lee H, Johnson ME. "Identification of B. anthracis N5-carboxyaminoimidazole ribonucleotide mutase (PurE) active site binding compounds via fragment library screening.". Bioorganic & medicinal chemistry. 2015;. doi:10.1016/j.bmc.2015.12.029.
  • Jain AD, Potteti H, Richardson BG, Kingsley L, Luciano JP, Ryuzoji AF, Lee H, Krunic A, Mesecar AD, Reddy SP, Moore T. "Probing the structural requirements of non-electrophilic naphthalene-based Nrf2 activators.". European journal of medicinal chemistry. 2015;103:252-68. doi:10.1016/j.ejmech.2015.08.049.
  • Rice AJ, Woo JK, Khan A, Szypulinski MZ, Johnson ME, Lee H, Lee H. "Over-expression, purification, and confirmation of Bacillus anthracis transcriptional regulator NprR.". Protein expression and purification. 2015;. doi:10.1016/j.pep.2015.08.030.
  • Han KY, Dugas-Ford J, Lee H, Chang JH, Azar DT. "MMP14 Cleavage of VEGFR1 in the Cornea Leads to a VEGF-Trap Antiangiogenic Effect.". Investigative ophthalmology & visual science. 2015;56(9):5450-6. doi:10.1167/iovs.14-16248.
  • Lee H, Lei H, Santarsiero BD, Gatuz JL, Cao S, Rice AJ, Patel K, Szypulinski MZ, Ojeda I, Ghosh AK, Johnson ME. "Inhibitor recognition specificity of MERS-CoV papain-like protease may differ from that of SARS-CoV.". ACS chemical biology. 2015;10(6):1456-65. doi:10.1021/cb500917m.
  • Aggarwal C, Jimenez Romaguera JC, Lee H, Chlipala GE, Ratia K, Federle M. "Identification of Quorum-Sensing Inhibitors Disrupting Signaling between Rgg and Short Hydrophobic Peptides in Streptococci.". mBio. 2015;6(3):e00393-15. doi:10.1128/mBio.00393-15.
  • Han KY, Chang M, Ying HY, Lee H, Huang YH, Chang JH, Azar DT. "Selective Binding of Endostatin Peptide 4 to Recombinant VEGF Receptor 3 In Vitro.". Protein and peptide letters. 2015;22(11):1025-30.
  • Lee H, Mittal A, Patel K, Gatuz JL, Truong L, Torres J, Mulhearn DC, Johnson ME. "Identification of novel drug scaffolds for inhibition of SARS-CoV 3-Chymotrypsin-like protease using virtual and high-throughput screenings.". Bioorganic & medicinal chemistry. 2014;22(1):167-77. doi:10.1016/j.bmc.2013.11.041.
  • Ratia K, Mehboob S, Lee H. "Ligand screening using enzymatic assays.". Methods in molecular biology (Clifton, N.J.). 2014;1140:291-304. doi:10.1007/978-1-4939-0354-2_21.
  • Rice AJ, Truong L, Johnson ME, Lee H. "A colorimetric assay optimization for high-throughput screening of dihydroorotase by detecting ureido groups.". Analytical biochemistry. 2013;441(1):87-94. doi:10.1016/j.ab.2013.05.035.
  • Lee H, Cao S, Hevener KE, Truong L, Gatuz JL, Patel K, Ghosh AK, Johnson ME. "Synergistic inhibitor binding to the papain-like protease of human SARS coronavirus: mechanistic and inhibitor design implications.". ChemMedChem. 2013;8(8):1361-72. doi:10.1002/cmdc.201300134.
  • Zhu T, Lee H, Lei H, Jones C, Patel K, Johnson ME, Hevener KE. "Fragment-based drug discovery using a multidomain, parallel MD-MM/PBSA screening protocol.". Journal of chemical information and modeling. 2013;53(3):560-72. doi:10.1021/ci300502h.
  • Truong L, Hevener KE, Rice AJ, Patel K, Johnson ME, Lee H. "High-level expression, purification, and characterization of Staphylococcus aureus dihydroorotase (PyrC) as a cleavable His-SUMO fusion.". Protein expression and purification. 2013;88(1):98-106. doi:10.1016/j.pep.2012.11.018.
  • Lee H, Zhu T, Patel K, Zhang YY, Truong L, Hevener KE, Gatuz JL, Subramanya G, Jeong H, Uprichard SL, Johnson ME. "High-throughput screening (HTS) and hit validation to identify small molecule inhibitors with activity against NS3/4A proteases from multiple hepatitis C virus genotypes.". PloS one. 2013;8(10):e75144. doi:10.1371/journal.pone.0075144.
  • Han KY, Azar DT, Sabri A, Lee H, Jain S, Lee BS, Chang JH. "Characterization of the interaction between endostatin short peptide and VEGF receptor 3.". Protein and peptide letters. 2012;19(9):969-74.
  • Chaudhuri R, Lee H, Truong L, Torres J, Patel K, Johnson ME. "Identification of non-macrocyclic small molecule inhibitors against the NS3/4A serine protease of hepatitis C virus through in silico screening.". Journal of chemical information and modeling. 2012;52(8):2245-56. doi:10.1021/ci300177p.
  • Lee H, Torres J, Truong L, Chaudhuri R, Mittal A, Johnson ME. "Reducing agents affect inhibitory activities of compounds: results from multiple drug targets.". Analytical biochemistry. 2012;423(1):46-53. doi:10.1016/j.ab.2012.01.006.
  • Ghosh AK, Takayama J, Rao KV, Ratia K, Chaudhuri R, Mulhearn DC, Lee H, Nichols DB, Baliji S, Baker SC, Johnson ME, Mesecar AD. "Severe acute respiratory syndrome coronavirus papain-like novel protease inhibitors: design, synthesis, protein-ligand X-ray structure and biological evaluation.". Journal of medicinal chemistry. 2010;53(13):4968-79. doi:10.1021/jm1004489.
  • Mahajan A, Yuan C, Lee H, Chen ES, Wu PY, Tsai MD. "Structure and function of the phosphothreonine-specific FHA domain.". Science signaling. 2008;1(51):re12. doi:10.1126/scisignal.151re12.
  • Lee H, Yuan C, Hammet A, Mahajan A, Chen ES, Wu MR, Su MI, Heierhorst J, Tsai MD. "Diphosphothreonine-specific interaction between an SQ/TQ cluster and an FHA domain in the Rad53-Dun1 kinase cascade.". Molecular cell. 2008;30(6):767-78. doi:10.1016/j.molcel.2008.05.013.
  • Qin D, Lee H, Yuan C, Ju Y, Tsai MD. "Identification of potential binding sites for the FHA domain of human Chk2 by in vitro binding studies.". Biochemical and biophysical research communications. 2003;311(4):803-8.
  • Lee H, Mok KH, Muhandiram R, Park KH, Suk JE, Kim DH, Chang J, Sung YC, Choi KY, Han KH. "Local structural elements in the mostly unstructured transcriptional activation domain of human p53.". The Journal of biological chemistry. 2000;275(38):29426-32. doi:10.1074/jbc.M003107200.

Review Hyun Lee’s online CV