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Photo of Michael (Mike) E Johnson

Michael (Mike) E Johnson

Professor Emeritus, Center for Biomolecular Sciences

Professor Emeritus, Medicinal Chemistry and Pharmacognosy

Contact Information

900 S. Ashland (870)
MBRB 3072
Chicago, IL 60607-7173

Phone: (312) 996-9114

Fax: (312) 413-9303


Lab Phone: (312) 413-9304

Intellectual Contributions

Journal Article

  • Lei H, Jones C, Zhu T, Patel K, Wolf NM, Fung LW, Lee H, Johnson ME. "Identification of B. anthracis N5-carboxyaminoimidazole ribonucleotide mutase (PurE) active site binding compounds via fragment library screening.". Bioorganic & medicinal chemistry. 2015;. doi:10.1016/j.bmc.2015.12.029.
  • McClay K, Mehboob S, Yu J, Santarsiero BD, Deng J, Cook JL, Jeong H, Johnson ME, Steffan RJ. "Indole trimers with antibacterial activity against Gram-positive organisms produced using combinatorial biocatalysis.". AMB Express. 2015;5(1):125. doi:10.1186/s13568-015-0125-4.
  • Rice AJ, Woo JK, Khan A, Szypulinski MZ, Johnson ME, Lee H, Lee H. "Over-expression, purification, and confirmation of Bacillus anthracis transcriptional regulator NprR.". Protein expression and purification. 2015;. doi:10.1016/j.pep.2015.08.030.
  • Lee H, Lei H, Santarsiero BD, Gatuz JL, Cao S, Rice AJ, Patel K, Szypulinski MZ, Ojeda I, Ghosh AK, Johnson ME. "Inhibitor recognition specificity of MERS-CoV papain-like protease may differ from that of SARS-CoV.". ACS chemical biology. 2015;10(6):1456-65. doi:10.1021/cb500917m.
  • Mehboob S, Song J, Hevener KE, Su P, Boci T, Brubaker L, Truong L, Mistry TL, Deng J, Cook JL, Santarsiero BD, Ghosh AK, Johnson ME. "Structural and biological evaluation of a novel series of benzimidazole inhibitors of Francisella tularensis enoyl-ACP reductase (FabI).". Bioorganic & medicinal chemistry letters. 2015;25(6):1292-6. doi:10.1016/j.bmcl.2015.01.048.
  • Tuntland ML, Santarsiero BD, Johnson ME, Fung LW. "Elucidation of the bicarbonate binding site and insights into the carboxylation mechanism of (N(5))-carboxyaminoimidazole ribonucleotide synthase (PurK) from Bacillus anthracis.". Acta crystallographica. Section D, Biological crystallography. 2014;70(Pt 11):3057-65. doi:10.1107/S1399004714021166.
  • Zhang YY, Mehboob S, Song JH, Boci T, Johnson ME, Ghosh AK, Jeong H. "Metabolism-directed structure optimization of benzimidazole-based Francisella tularensis enoyl-reductase (FabI) inhibitors.". Xenobiotica; the fate of foreign compounds in biological systems. 2014;44(5):404-16. doi:10.3109/00498254.2013.850553.
  • Lee H, Mittal A, Patel K, Gatuz JL, Truong L, Torres J, Mulhearn DC, Johnson ME. "Identification of novel drug scaffolds for inhibition of SARS-CoV 3-Chymotrypsin-like protease using virtual and high-throughput screenings.". Bioorganic & medicinal chemistry. 2014;22(1):167-77. doi:10.1016/j.bmc.2013.11.041.
  • Rice AJ, Truong L, Johnson ME, Lee H. "A colorimetric assay optimization for high-throughput screening of dihydroorotase by detecting ureido groups.". Analytical biochemistry. 2013;441(1):87-94. doi:10.1016/j.ab.2013.05.035.
  • Lee H, Cao S, Hevener KE, Truong L, Gatuz JL, Patel K, Ghosh AK, Johnson ME. "Synergistic inhibitor binding to the papain-like protease of human SARS coronavirus: mechanistic and inhibitor design implications.". ChemMedChem. 2013;8(8):1361-72. doi:10.1002/cmdc.201300134.
  • Zhu T, Lee H, Lei H, Jones C, Patel K, Johnson ME, Hevener KE. "Fragment-based drug discovery using a multidomain, parallel MD-MM/PBSA screening protocol.". Journal of chemical information and modeling. 2013;53(3):560-72. doi:10.1021/ci300502h.
  • Truong L, Hevener KE, Rice AJ, Patel K, Johnson ME, Lee H. "High-level expression, purification, and characterization of Staphylococcus aureus dihydroorotase (PyrC) as a cleavable His-SUMO fusion.". Protein expression and purification. 2013;88(1):98-106. doi:10.1016/j.pep.2012.11.018.
  • Lee H, Zhu T, Patel K, Zhang YY, Truong L, Hevener KE, Gatuz JL, Subramanya G, Jeong H, Uprichard SL, Johnson ME. "High-throughput screening (HTS) and hit validation to identify small molecule inhibitors with activity against NS3/4A proteases from multiple hepatitis C virus genotypes.". PloS one. 2013;8(10):e75144. doi:10.1371/journal.pone.0075144.
  • Hevener KE, Mehboob S, Boci T, Truong K, Santarsiero BD, Johnson ME. "Expression, purification and characterization of enoyl-ACP reductase II, FabK, from Porphyromonas gingivalis.". Protein expression and purification. 2012;85(1):100-8. doi:10.1016/j.pep.2012.07.003.
  • Chaudhuri R, Lee H, Truong L, Torres J, Patel K, Johnson ME. "Identification of non-macrocyclic small molecule inhibitors against the NS3/4A serine protease of hepatitis C virus through in silico screening.". Journal of chemical information and modeling. 2012;52(8):2245-56. doi:10.1021/ci300177p.
  • Mehboob S, Hevener KE, Truong K, Boci T, Santarsiero BD, Johnson ME. "Structural and enzymatic analyses reveal the binding mode of a novel series of Francisella tularensis enoyl reductase (FabI) inhibitors.". Journal of medicinal chemistry. 2012;55(12):5933-41. doi:10.1021/jm300489v.
  • Lee H, Torres J, Truong L, Chaudhuri R, Mittal A, Johnson ME. "Reducing agents affect inhibitory activities of compounds: results from multiple drug targets.". Analytical biochemistry. 2012;423(1):46-53. doi:10.1016/j.ab.2012.01.006.
  • Tuntland ML, Johnson ME, Fung LW, Santarsiero BD. "Structure of N5-carboxyaminoimidazole ribonucleotide synthase (PurK) from Bacillus anthracis.". Acta crystallographica. Section D, Biological crystallography. 2011;67(Pt 10):870-4. doi:10.1107/S0907444911029210.
  • Mehboob S, Mulhearn DC, Truong K, Johnson ME, Santarsiero BD. "Structure of dihydroorotase from Bacillus anthracis at 2.6 Å resolution.". Acta crystallographica. Section F, Structural biology and crystallization communications. 2010;66(Pt 11):1432-5. doi:10.1107/S1744309110037085.
  • Mehboob S, Truong K, Santarsiero BD, Johnson ME. "Structure of the Francisella tularensis enoyl-acyl carrier protein reductase (FabI) in complex with NAD(+) and triclosan.". Acta crystallographica. Section F, Structural biology and crystallization communications. 2010;66(Pt 11):1436-40. doi:10.1107/S1744309110039862.
  • Ghosh AK, Takayama J, Rao KV, Ratia K, Chaudhuri R, Mulhearn DC, Lee H, Nichols DB, Baliji S, Baker SC, Johnson ME, Mesecar AD. "Severe acute respiratory syndrome coronavirus papain-like novel protease inhibitors: design, synthesis, protein-ligand X-ray structure and biological evaluation.". Journal of medicinal chemistry. 2010;53(13):4968-79. doi:10.1021/jm1004489.
  • Mehboob S, Song Y, Witek M, Long F, Santarsiero BD, Johnson ME, Fung LW. "Crystal structure of the nonerythroid alpha-spectrin tetramerization site reveals differences between erythroid and nonerythroid spectrin tetramer formation.". The Journal of biological chemistry. 2010;285(19):14572-84. doi:10.1074/jbc.M109.080028.
  • Tipparaju SK, Mulhearn DC, Klein GM, Chen Y, Tapadar S, Bishop MH, Yang S, Chen J, Ghassemi M, Santarsiero BD, Cook JL, Johlfs M, Mesecar AD, Johnson ME, Kozikowski AP. "Design and synthesis of aryl ether inhibitors of the Bacillus anthracis enoyl-ACP reductase.". ChemMedChem. 2008;3(8):1250-68. doi:10.1002/cmdc.200800047.
  • May M, Mehboob S, Mulhearn DC, Wang Z, Yu H, Thatcher GR, Santarsiero BD, Johnson ME, Mesecar AD. "Structural and functional analysis of two glutamate racemase isozymes from Bacillus anthracis and implications for inhibitor design.". Journal of molecular biology. 2007;371(5):1219-37. doi:10.1016/j.jmb.2007.05.093.
  • Ghosh AK, Xi K, Ratia K, Santarsiero BD, Fu W, Harcourt BH, Rota PA, Baker SC, Johnson ME, Mesecar AD. "Design and synthesis of peptidomimetic severe acute respiratory syndrome chymotrypsin-like protease inhibitors.". Journal of medicinal chemistry. 2005;48(22):6767-71. doi:10.1021/jm050548m.
  • Park S, Hayes BL, Marankan F, Mulhearn DC, Wanna L, Mesecar AD, Santarsiero BD, Johnson ME, Venton DL. "Regioselective covalent modification of hemoglobin in search of antisickling agents.". Journal of medicinal chemistry. 2003;46(6):936-53. doi:10.1021/jm020361k.