Photo of Professor Mike E Johnson

Mike E Johnson

Professor Emeritus, Center for Biomolecular Sciences
Professor Emeritus, Pharmaceutical Sciences


Building & Room:

MBRB 3072


900 S. Ashland, Chicago, IL, 60607-7173

Office Phone:

(312) 996-9114

Related Sites:

Office Phone:

(312) 413-9304

Selected Publications

  • Lei HM, Jones CW, Zhu T, Patel KE, Wolf N, Fung L, Lee H, Johnson M. Identification of B. anthracis N5-carboxyaminoimidazole ribonucleotide mutase (PurE) active site binding compounds via fragment library screening.. Bioorganic & medicinal chemistry. 2015;. doi:10.1016/j.bmc.2015.12.029.
  • McClay KD, Mehboob SL, Yu J, Santarsiero BE, Deng JJ, Cook J, Jeong H, Johnson M, Steffan R. Indole trimers with antibacterial activity against Gram-positive organisms produced using combinatorial biocatalysis.. AMB Express. 2015;5(1):125. doi:10.1186/s13568-015-0125-4.
  • Rice AJ, Woo JK, Khan AZ, Szypulinski ME, Johnson M, Lee H, Lee H. Over-expression, purification, and confirmation of Bacillus anthracis transcriptional regulator NprR.. Protein expression and purification. 2015;. doi:10.1016/j.pep.2015.08.030.
  • Lee H, Lei HD, Santarsiero BL, Gatuz JJ, Cao SZ, Rice AK, Patel KE, Szypulinski M, Ojeda I, Ghosh A, Johnson M. Inhibitor recognition specificity of MERS-CoV papain-like protease may differ from that of SARS-CoV.. ACS chemical biology. 2015;10(6):1456-65. doi:10.1021/cb500917m.
  • Mehboob SE, Song J, Hevener KL, Su PL, Boci TD, Brubaker LK, Truong LE, Mistry T, Deng J, Cook J, Santarsiero B, Ghosh A, Johnson M. Structural and biological evaluation of a novel series of benzimidazole inhibitors of Francisella tularensis enoyl-ACP reductase (FabI).. Bioorganic & medicinal chemistry letters. 2015;25(6):1292-6. doi:10.1016/j.bmcl.2015.01.048.
  • Tuntland ML, Santarsiero BD, Johnson ME, Fung LW. Elucidation of the bicarbonate binding site and insights into the carboxylation mechanism of (N(5))-carboxyaminoimidazole ribonucleotide synthase (PurK) from Bacillus anthracis.. Acta crystallographica. Section D, Biological crystallography. 2014;70(Pt 11):3057-65. doi:10.1107/S1399004714021166.
  • Zhang YY, Mehboob SH, Song JE, Boci TK, Johnson M, Ghosh A, Jeong H. Metabolism-directed structure optimization of benzimidazole-based Francisella tularensis enoyl-reductase (FabI) inhibitors.. Xenobiotica; the fate of foreign compounds in biological systems. 2014;44(5):404-16. doi:10.3109/00498254.2013.850553.
  • Lee H, Mittal AL, Patel KC, Gatuz JE, Truong L, Torres J, Mulhearn D, Johnson M. Identification of novel drug scaffolds for inhibition of SARS-CoV 3-Chymotrypsin-like protease using virtual and high-throughput screenings.. Bioorganic & medicinal chemistry. 2014;22(1):167-77. doi:10.1016/j.bmc.2013.11.041.
  • Rice AJ, Truong LE, Johnson M, Lee H. A colorimetric assay optimization for high-throughput screening of dihydroorotase by detecting ureido groups.. Analytical biochemistry. 2013;441(1):87-94. doi:10.1016/j.ab.2013.05.035.
  • Lee H, Cao SE, Hevener KL, Truong LK, Gatuz JE, Patel K, Ghosh A, Johnson M. Synergistic inhibitor binding to the papain-like protease of human SARS coronavirus: mechanistic and inhibitor design implications.. ChemMedChem. 2013;8(8):1361-72. doi:10.1002/cmdc.201300134.
  • Zhu T, Lee HE, Lei HE, Jones C, Patel K, Johnson M, Hevener K. Fragment-based drug discovery using a multidomain, parallel MD-MM/PBSA screening protocol.. Journal of chemical information and modeling. 2013;53(3):560-72. doi:10.1021/ci300502h.
  • Truong LE, Hevener KJ, Rice AE, Patel K, Johnson M, Lee H. High-level expression, purification, and characterization of Staphylococcus aureus dihydroorotase (PyrC) as a cleavable His-SUMO fusion.. Protein expression and purification. 2013;88(1):98-106. doi:10.1016/j.pep.2012.11.018.
  • Lee H, Zhu TY, Patel KE, Zhang YL, Truong L, Hevener KL, Gatuz JE, Subramanya G, Jeong H, Uprichard S, Johnson M. High-throughput screening (HTS) and hit validation to identify small molecule inhibitors with activity against NS3/4A proteases from multiple hepatitis C virus genotypes.. PloS one. 2013;8(10):e75144. doi:10.1371/journal.pone.0075144.
  • Hevener KE, Mehboob SD, Boci TE, Truong K, Santarsiero B, Johnson M. Expression, purification and characterization of enoyl-ACP reductase II, FabK, from Porphyromonas gingivalis.. Protein expression and purification. 2012;85(1):100-8. doi:10.1016/j.pep.2012.07.003.
  • Chaudhuri R, Lee HE, Truong L, Torres J, Patel K, Johnson M. Identification of non-macrocyclic small molecule inhibitors against the NS3/4A serine protease of hepatitis C virus through in silico screening.. Journal of chemical information and modeling. 2012;52(8):2245-56. doi:10.1021/ci300177p.
  • Mehboob SE, Hevener KD, Truong KE, Boci T, Santarsiero B, Johnson M. Structural and enzymatic analyses reveal the binding mode of a novel series of Francisella tularensis enoyl reductase (FabI) inhibitors.. Journal of medicinal chemistry. 2012;55(12):5933-41. doi:10.1021/jm300489v.
  • Lee H, Torres JE, Truong L, Chaudhuri R, Mittal A, Johnson M. Reducing agents affect inhibitory activities of compounds: results from multiple drug targets.. Analytical biochemistry. 2012;423(1):46-53. doi:10.1016/j.ab.2012.01.006.
  • Tuntland ML, Johnson ME, Fung LW, Santarsiero BD. Structure of N5-carboxyaminoimidazole ribonucleotide synthase (PurK) from Bacillus anthracis.. Acta crystallographica. Section D, Biological crystallography. 2011;67(Pt 10):870-4. doi:10.1107/S0907444911029210.
  • Mehboob SC, Mulhearn DE, Truong KD, Johnson M, Santarsiero B. Structure of dihydroorotase from Bacillus anthracis at 2.6 Å resolution.. Acta crystallographica. Section F, Structural biology and crystallization communications. 2010;66(Pt 11):1432-5. doi:10.1107/S1744309110037085.
  • Mehboob SD, Truong KE, Santarsiero B, Johnson M. Structure of the Francisella tularensis enoyl-acyl carrier protein reductase (FabI) in complex with NAD(+) and triclosan.. Acta crystallographica. Section F, Structural biology and crystallization communications. 2010;66(Pt 11):1436-40. doi:10.1107/S1744309110039862.
  • Ghosh AK, Takayama JV, Rao KC, Ratia K, Chaudhuri RB, Mulhearn DC, Lee HE, Nichols DD, Baliji S, Baker S, Johnson M, Mesecar A. Severe acute respiratory syndrome coronavirus papain-like novel protease inhibitors: design, synthesis, protein-ligand X-ray structure and biological evaluation.. Journal of medicinal chemistry. 2010;53(13):4968-79. doi:10.1021/jm1004489.
  • Mehboob SD, Song YE, Witek MW, Long F, Santarsiero B, Johnson M, Fung L. Crystal structure of the nonerythroid alpha-spectrin tetramerization site reveals differences between erythroid and nonerythroid spectrin tetramer formation.. The Journal of biological chemistry. 2010;285(19):14572-84. doi:10.1074/jbc.M109.080028.
  • Tipparaju SK, Mulhearn DC, Klein GM, Chen YH, Tapadar SD, Bishop ML, Yang SD, Chen JE, Ghassemi MP, Santarsiero B, Cook J, Johlfs M, Mesecar A, Johnson M, Kozikowski A. Design and synthesis of aryl ether inhibitors of the Bacillus anthracis enoyl-ACP reductase.. ChemMedChem. 2008;3(8):1250-68. doi:10.1002/cmdc.200800047.
  • May MC, Mehboob S, Mulhearn DR, Wang ZD, Yu HE, Thatcher GD, Santarsiero B, Johnson M, Mesecar A. Structural and functional analysis of two glutamate racemase isozymes from Bacillus anthracis and implications for inhibitor design.. Journal of molecular biology. 2007;371(5):1219-37. doi:10.1016/j.jmb.2007.05.093.
  • Ghosh AK, Xi KD, Ratia KH, Santarsiero BA, Fu WC, Harcourt BE, Rota PD, Baker S, Johnson M, Mesecar A. Design and synthesis of peptidomimetic severe acute respiratory syndrome chymotrypsin-like protease inhibitors.. Journal of medicinal chemistry. 2005;48(22):6767-71. doi:10.1021/jm050548m.
  • Park SL, Hayes BC, Marankan FD, Mulhearn DD, Wanna LE, Mesecar AL, Santarsiero B, Johnson M, Venton D. Regioselective covalent modification of hemoglobin in search of antisickling agents.. Journal of medicinal chemistry. 2003;46(6):936-53. doi:10.1021/jm020361k.