Alessandra Eustaquio
Assistant Professor, College of Pharmacy
Email:
Related Sites:
About
Teaching and Supervision
Doctoral Research in Pharmacog (PMPG 599), 6/14/2021 – 8/6/2021
Doctoral Research in Pharmacog (PMPG 599), 1/11/2021 – 4/30/2021
Biomedicinal Chemistry (PHAR 423), 1/11/2021 – 4/30/2021
Biomedicinal Chemistry (PHAR 423), 1/11/2021 – 4/30/2021
Pharmacog Research Techniques (PSCI 520), 8/24/2020 – 12/4/2020
Pharm Genomics Bioinformatics (PMMP 412), 8/24/2020 – 12/4/2020
Pharm Genomics Bioinformatics (PMMP 412), 8/24/2020 – 12/4/2020
Doctoral Research in Pharmacog (PMPG 599), 8/24/2020 – 12/4/2020
Doctoral Research in Pharmacog (PMPG 599), 6/15/2020 – 8/7/2020
Selected Grants
CCTS KL2 CATS Scholar, NIH CCTS KL2 institutional grant., 6/1/2021 - 5/31/2019, No Obligated Amount Set; No Anticipated Amount Set
Incoming MTA: Development of a Bacterial Host for Natural Product Discovery and Production, Addgene., 1/1/2021 - 12/31/2026, No Obligated Amount Set; No Anticipated Amount Set
★ Development of a bacterial host for natural product discovery and production, National Institutes of Health (National Institute of General Medical Sciences)., 9/20/2020 - 6/30/2024, Obligated Amount: $793438; Anticipated Amount: $1532645
★ Discovery of Anticancer Agents of Diverse Natural Origin, Ohio State University., 5/15/2020 - 4/30/2022, Obligated Amount: $674076; Anticipated Amount: $674076
★ Role of a conserved gene cluster family in promoting motility behavior in proteobacteria and in mediating host interactions, National Science Foundation., 8/15/2019 - 7/31/2022, Obligated Amount: $546571; Anticipated Amount: $546571
Hans Vahlteich Research Scholar., 1/1/2016 - Present, No Obligated Amount Set; No Anticipated Amount Set
Discovery of anticancer agents of diverse natural origin, National Institutes of Health., 12/1/2006 - 4/30/2020, No Obligated Amount Set; No Anticipated Amount Set
Selected Publications
Ióca, LP, Dai, Y, Kunakom, S, Diaz-Espinosa, J, Krunic, A, Crnkovic, CM, Orjala, J, Sanchez, LM, Ferreira, AG, Berlinck, RGS, Eustáquio, AS. (2021). A Family of Nonribosomal Peptides Modulate Collective Behavior in Pseudovibrio Bacteria Isolated from Marine Sponges**. Angewandte Chemie – International Edition, 60, (29), 15891-15898. doi:10.1002/anie.202017320.
★ Kornfuehrer, T, Romanowski, S, de Crécy-Lagard, V, Hanson, AD, Eustáquio, AS. (2020). An Enzyme Containing the Conserved Domain of Unknown Function DUF62 Acts as a Stereoselective (Rs,Sc)-S-Adenosylmethionine Hydrolase. ChemBioChem, 21, (24), 3495-3499. doi:10.1002/cbic.202000349.
★ Romanowski, S, Eustáquio, AS. (2020). Synthetic biology for natural product drug production and engineering. Current Opinion in Chemical Biology, 58, 137-145. doi:10.1016/j.cbpa.2020.09.006.
★ Crnkovic, CM, Braesel, J, Krunic, A, Eustáquio, AS, Orjala, J. (2020). Scytodecamide from the Cultured Scytonema sp. UIC 10036 Expands the Chemical and Genetic Diversity of Cyanobactins. ChemBioChem, 21, (6), 845-852. doi:10.1002/cbic.201900511.
★ Kunakom, S, Eustáquio, AS. (2020). Heterologous Production of Lasso Peptide Capistruin in a Burkholderia Host. ACS Synthetic Biology, 9, (2), 241-248. doi:10.1021/acssynbio.9b00438.
★ Braesel, J, Tran, TA, Eustáquio, AS. (2019). Heterologous expression of the diazaquinomycin biosynthetic gene cluster. Journal of Industrial Microbiology and Biotechnology, 46, (9-10), 1359-1364. doi:10.1007/s10295-019-02187-1.
★ Kunakom, S, Eustáquio, AS. (2019). Burkholderia as a Source of Natural Products. Journal of Natural Products, 82, (7), 2018-2037. doi:10.1021/acs.jnatprod.8b01068.
★ Braesel, J, Clark, CM, Kunstman, KJ, Green, SJ, Maienschein-Cline, M, Murphy, BT, Eustáquio, AS. (2019). Genome sequence of marine-derived Streptomyces sp. Strain F001, a producer of Akashin A and diazaquinomycins. Microbiology Resource Announcements, 8, (19). doi:10.1128/MRA.00165-19.
★ Kunakom, S, Eustáquio, AS. (2019). Natural products and synthetic biology: Where we are and where we need to go. mSystems, 4, (3). doi:10.1128/mSystems.00113-19.
★ Braesel, J, Lee, JH, Arnould, B, Murphy, BT, Eustáquio, AS. (2019). Diazaquinomycin Biosynthetic Gene Clusters from Marine and Freshwater Actinomycetes. Journal of Natural Products, 82, (4), 937-946. doi:10.1021/acs.jnatprod.8b01028.
★ Braesel, J, Eustáquio, AS. (2019). Heterologous expression of a putative ClpC chaperone gene leads to induction of a host metabolite. Journal of the Brazilian Chemical Society, 30, (3), 499-508. doi:10.21577/0103-5053.20180234.
★ Kornfuehrer, T, Eustáquio, AS. (2019). Diversification of polyketide structures: Via synthase engineering. MedChemComm, 10, (8), 1256-1272. doi:10.1039/c9md00141g.
★ Braesel, J, Crnkovic, CM, Kunstman, KJ, Green, SJ, Maienschein-Cline, M, Orjala, J, Murphy, BT, Eustáquio, AS. (2018). Complete Genome of Micromonospora sp. Strain B006 Reveals Biosynthetic Potential of a Lake Michigan Actinomycete. Journal of Natural Products, 81, (9), 2057-2068. doi:10.1021/acs.jnatprod.8b00394.
★ Eustáquio, Alessandra S, Ziemert, Nadine. (2018). Identification of Natural Product Biosynthetic Gene Clusters from Bacterial Genomic Data. (pp. 1-21). doi:10.1007/7653_2018_32.
Muhammad, Maryam B, Eustaquio, Alessandra S. (2018). Review of Chemistry in the Marketplace. Journal of Natural Products, 81, (4), 1127-1127. doi:10.1021/acs.jnatprod.8b00109.
Davis, TD, Kunakom, S, Burkart, MD, Eustaquio, AS. (2018). Preparation, Assay, and Application of Chlorinase SalL for the Chemoenzymatic Synthesis of S-Adenosyl-L-Methionine and Analogs. Methods in Enzymology. (pp. 367-388). doi:10.1016/bs.mie.2018.02.012.
★ May, DS, Chen, WL, Lantvit, DD, Zhang, X, Krunic, A, Burdette, JE, Eustaquio, A, Orjala, J. (2017). Merocyclophanes C and D from the Cultured Freshwater Cyanobacterium Nostoc sp. (UIC 10110). Journal of Natural Products, 80, (4), 1073-1080. doi:10.1021/acs.jnatprod.6b01175.
Agarwal, V, Miles, ZD, Winter, JM, Eustáquio, AS, El Gamal, AA, Moore, BS. (2017). Enzymatic Halogenation and Dehalogenation Reactions: Pervasive and Mechanistically Diverse. Chemical Reviews, 117, (8), 5619-5674. doi:10.1021/acs.chemrev.6b00571.
Nicacio, KJ, Ióca, LP, Fróes, AM, Leomil, L, Appolinario, LR, Thompson, CC, Thompson, FL, Ferreira, AG, Williams, DE, Andersen, RJ, Eustaquio, AS, Berlinck, RGS. (2017). Cultures of the Marine Bacterium Pseudovibrio denitrificans Ab134 Produce Bromotyrosine-Derived Alkaloids Previously Only Isolated from Marine Sponges. Journal of Natural Products, 80, (2), 235-240. doi:10.1021/acs.jnatprod.6b00838.
Eustáquio, AS, Chang, LP, Steele, GL, O’Donnell, CJ, Koehn, FE. (2016). Biosynthetic engineering and fermentation media development leads to gram-scale production of spliceostatin natural products in Burkholderia sp.. Metabolic Engineering, 33, 67-75. doi:10.1016/j.ymben.2015.11.003.
Eustáquio, AS, Janso, JE, Ratnayake, AS, O’Donnell, CJ, Koehn, FE. (2014). Spliceostatin hemiketal biosynthesis in Burkholderia spp. is catalyzed by an iron/α-ketoglutarate-dependent dioxygenase. Proceedings of the National Academy of Sciences of the United States of America, 111, (33). doi:10.1073/pnas.1408300111.
Janso, JE, Haltli, BA, Eustáquio, AS, Kulowski, K, Waldman, AJ, Zha, L, Nakamura, H, Bernan, VS, He, H, Carter, GT, Koehn, FE, Balskus, EP. (2014). Discovery of the lomaiviticin biosynthetic gene cluster in Salinispora pacifica. Tetrahedron, 70, (27-28), 4156-4164. doi:10.1016/j.tet.2014.03.009.
Jones, AC, Ottilie, S, Eustáquio, AS, Edwards, DJ, Gerwick, L, Moore, BS, Gerwick, WH. (2012). Evaluation of Streptomyces coelicolor A3(2) as a heterologous expression host for the cyanobacterial protein kinase C activator lyngbyatoxin A. FEBS Journal, 279, (7), 1243-1251. doi:10.1111/j.1742-4658.2012.08517.x.
Lechner, A, Eustáquio, AS, Gulder, TAM, Hafner, M, Moore, BS. (2011). Selective overproduction of the proteasome inhibitor salinosporamide A via precursor pathway regulation. Chemistry and Biology, 18, (12), 1527-1536. doi:10.1016/j.chembiol.2011.10.014.
Miyanaga, A, Janso, JE, McDonald, L, He, M, Liu, H, Barbieri, L, Eustáquio, AS, Fielding, EN, Carter, GT, Jensen, PR, Feng, X, Leighton, M, Koehn, FE, Moore, BS. (2011). Discovery and assembly-line biosynthesis of the lymphostin pyrroloquinoline alkaloid family of mtor inhibitors in salinispora bacteria. Journal of the American Chemical Society, 133, (34), 13311-13313. doi:10.1021/ja205655w.
Udwary, DW, Gontang, EA, Jones, AC, Jones, CS, Schultz, AW, Winter, JM, Yang, JY, Beauchemin, N, Capson, TL, Clark, BR, Esquenazi, E, Eustáquio, AS, Freel, K, Gerwick, L, Gerwick, WH, Gonzalez, D, Liu, WT, Malloy, KL, Maloney, KN, Nett, M, Nunnery, JK, Penn, K, Prieto-Davo, A, Simmons, TL, Weitz, S, Wilson, MC, Tisa, LS, Dorrestein, PC, Moore, BS. (2011). Significant natural product biosynthetic potential of actinorhizal symbionts of the genus Frankia, as revealed by comparative genomic and proteomic analyses. Applied and Environmental Microbiology, 77, (11), 3617-3625. doi:10.1128/AEM.00038-11.
Eustáquio, AS, Nam, SJ, Penn, K, Lechner, A, Wilson, MC, Fenical, W, Jensen, PR, Moore, BS. (2011). The discovery of salinosporamide K from the marine bacterium “Salinispora pacifica” by Genome mining gives insight into pathway evolution. ChemBioChem, 12, (1), 61-64. doi:10.1002/cbic.201000564.
Eustáquio, AS, O’Hagan, D, Moore, BS. (2010). Engineering fluorometabolite production: Fluorinase expression in salinispora tropica yields fluorosalinosporamide. Journal of Natural Products, 73, (3), 378-382. doi:10.1021/np900719u.
Deng, H, McMahon, SA, Eustáquio, AS, Moore, BS, Naismith, JH, O’Hagan, D. (2009). Mechanistic insights into water activation in SAM hydroxide adenosyltransferase (duf-62). ChemBioChem, 10, (15), 2455-2459. doi:10.1002/cbic.200900369.
Liu, Y, Hazzard, C, Eustáquio, AS, Reynolds, KA, Moore, BS. (2009). Biosynthesis of salinosporamides from α,β-unsaturated fatty acids: Implications for extending polyketide synthase diversity. Journal of the American Chemical Society, 131, (30), 10376-10377. doi:10.1021/ja9042824.
Eustáquio, AS, McGlinchey, RP, Liu, Y, Hazzard, C, Beer, LL, Florova, G, Alhamadsheh, MM, Lechner, A, Kale, AJ, Kobayashi, Y, Reynolds, KA, Moore, BS. (2009). Biosynthesis of the salinosporamide A polyketide synthase substrate chloroethylmalonyl-coenzyme A from S-adenosyl-L-methionine. Proceedings of the National Academy of Sciences of the United States of America, 106, (30), 12295-12300. doi:10.1073/pnas.0901237106.
Dangel, V, Eustáquio, AS, Gust, B, Heide, L. (2008). novE and novG act as positive regulators of novobiocin biosynthesis. Archives of Microbiology, 190, (5), 509-519. doi:10.1007/s00203-008-0396-0.
Eustáquio, AS, Härle, J, Noel, JP, Moore, BS. (2008). S-adenosyl-L-methionine hydrolase (adenosine-forming), a conserved bacterial and archeal protein related to SAM-dependent halogenases. ChemBioChem, 9, (14), 2215-2219. doi:10.1002/cbic.200800341.
Moore, BS, Eustáquio, AS, McGlinchey, RP. (2008). Advances in and applications of proteasome inhibitors. Current Opinion in Chemical Biology, 12, (4), 434-440. doi:10.1016/j.cbpa.2008.06.033.
McGlinchey, RP, Nett, M, Eustáquio, AS, Asolkar, RN, Fenical, W, Moore, BS. (2008). Engineered biosynthesis of antiprotealide and other unnatural salinosporamide proteasome inhibitors. Journal of the American Chemical Society, 130, (25), 7822-7823. doi:10.1021/ja8029398.
Eustáquio, AS, Moore, BS. (2008). Mutasynthesis of fluorosalinosporamide, a potent and reversible inhibitor of the proteasome. Angewandte Chemie – International Edition, 47, (21), 3936-3938. doi:10.1002/anie.200800177.
Eustáquio, AS, Pojer, F, Noel, JP, Moore, BS. (2008). Discovery and characterization of a marine bacterial SAM-dependent chlorinase. Nature Chemical Biology, 4, (1), 69-74. doi:10.1038/nchembio.2007.56.
Flatman, RH, Eustaquio, A, Li, SM, Heide, L, Maxwell, A. (2006). Structure-activity relationships of aminocoumarin-type gyrase and topoisomerase IV inhibitors obtained by combinatorial biosynthesis. Antimicrobial Agents and Chemotherapy, 50, (4), 1136-1142. doi:10.1128/AAC.50.4.1136-1142.2006.
Professional Leadership
American Society of Pharmacognosy auditing committee, 6/1/2020 - Present
ASP Broadening the Reviewer Pool Task Force, 6/1/2020 - Present
National Science Foundation - Scientific Review Committee, National Science Foundation, 2018 - 2019
National Institutes of Health review panel – Synthetic and Biological Chemistry B (SBCB) Study section, National Institutes of Health, 6/2017 - Present
American Society of Pharmacognosy Auditing Committee, American Society of Pharmacognosy, 2016 - Present
Notable Honors
2017, Clinical and Translational Sciences Scholar, University of Illinois at Chicago
2016, Hans W. Vahlteich Research Scholar Award, University of Illinois at Chicago
2014, Star Award, Sustainable Science Category, Pfizer (United States)
2012, Green Chemistry Award, Pfizer (United States)
2011, Individual Performance Awards, Pfizer (United States)
2006, Life Sciences Research Foundation Postdoctoral Fellowship, Life Sciences Research Foundation
2005, PhD Prize for Best Dissertation, University of Tübingen
2004, Publication Award, German Pharmaceutical Society
1999, Valedictorian, University of Sao Paulo
1998, Undergraduate Research Training Fellowship, National Council for Scientific and Technological Development
Education
Degrees:
Ph.D., University of Tübingen, Germany, 2004
B.Sc., University of Sao Paulo, Brazil, 1999
Postgraduate Training:
Postdoctoral Researcher - Life Sciences Research Foundation Fellow, University of California, San Diego, United States, 2009
Selected Presentations
Eustaquio, Alessandra. (2021 April 07). From DNA to Natural Products Through Synthetic Biology. ACS Spring 2020 National Meeting. virtual.