Photo of Les  Hanakahi

Les Hanakahi

Associate Professor, Pharmaceutical Sciences


Building & Room:



Rockford, IL, 61107

Office Phone:

(815) 395-5924

Office Phone:

(815) 395-5907

Selected Publications

  • L , Summerlin MB, Nitiss K, Nitiss JL, Hanakahi . “TDP1 is required for efficient non-homologous end joining in human cells”. DNA Repair. 2017;60:40-49.
  • Jiao C, Summerlin MB, Bruzik KS, Hanakahi L. Synthesis of Biotinylated Inositol Hexakisphosphate To Study DNA Double-Strand Break Repair and Affinity Capture of IP6-Binding Proteins.. Biochemistry. 2015;54(41):6312-22. doi:10.1021/acs.biochem.5b00642.
  • Heo JB, Li JJ, Summerlin MC, Hays AL, Katyal S, McKinnon P, Nitiss K, Nitiss J, Hanakahi L. TDP1 promotes assembly of non-homologous end joining protein complexes on DNA.. DNA Repair. 2015;30:28-37. doi:10.1016/j.dnarep.2015.03.003.
  • Slavoff SA, Heo J, Budnik BA, Hanakahi L, Saghatelian A. A human short open reading frame (sORF)-encoded polypeptide that stimulates DNA end joining.. The Journal of biological chemistry. 2014;289(16):10950-7. doi:10.1074/jbc.C113.533968.
  • Hanakahi L. Effect of the inositol polyphosphate InsP(6) on DNA-PK-dependent phosphorylation.. Molecular cancer research : MCR. 2011;9(10):1366-76. doi:10.1158/1541-7786.MCR-11-0230.
  • Jayaram S, Gilson T, Ehrlich ES, Yu XF, Ketner G, Hanakahi L. E1B 55k-independent dissociation of the DNA ligase IV/XRCC4 complex by E4 34k during adenovirus infection.. Virology. 2008;382(2):163-70. doi:10.1016/j.virol.2008.08.045.
  • Cheung JC, Salerno B, Hanakahi L. Evidence for an inositol hexakisphosphate-dependent role for Ku in mammalian nonhomologous end joining that is independent of its role in the DNA-dependent protein kinase.. Nucleic acids research. 2008;36(17):5713-26. doi:10.1093/nar/gkn572.
  • Jayaram S, Ketner G, Adachi N, Hanakahi L. Loss of DNA ligase IV prevents recognition of DNA by double-strand break repair proteins XRCC4 and XLF.. Nucleic acids research. 2008;36(18):5773-86. doi:10.1093/nar/gkn552.
  • Hanakahi L. 2-Step purification of the Ku DNA repair protein expressed in Escherichia coli.. Protein expression and purification. 2007;52(1):139-45. doi:10.1016/j.pep.2006.10.002.
  • Smeaton MB, Miller PS, Ketner G, Hanakahi L. Small-scale extracts for the study of nucleotide excision repair and non-homologous end joining.. Nucleic acids research. 2007;35(22):e152. doi:10.1093/nar/gkm974.
  • Notable Honors

    2014, Teacher of the Semester, P1 class

    2013, Vahlteich Research Scholar,


  • PhD, Biochemistry, Yale University, New Haven, CT
  • BS, Biology, University of Hawaii, Manoa, Honolulu, Hawaii
  • Professional Memberships

  • Evironmental Mutagen and Genomics Society