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Photo of John L. Nitiss

John L. Nitiss


Professor of Pharmacology, College of Pharmacy - Pharmaceutical Sciences
Associate Head for Research, College of Pharmacy - Pharmaceutical Sciences
Assistant Dean for Research, Rockford Campus, College of Pharmacy
Adjunct professor, College of Medicine - Medical Biotechnology

Contact

Address:

1601 Parkview Avenue, Rockford, IL

Office Phone:

(815) 395-5583 (work)

Related Sites:

About Heading link

Research Interests:
Mechanisms of action of anticancer drugs; DNA topoisomerases and their roles in biological processes; microbial model systems of drug action; biochemistry and biology of non-homologous recombination; repair inhibitors and cancer chemotherapy.

Teaching and Supervision Heading link

Undergrad Res Pharm Sci (PSCI 380), 1/9/2023 – 4/28/2023
Colloquium Lecture Series (PSCI 425), 8/22/2022 – 12/2/2022
Colloquium Lecture Series (PSCI 425), 8/22/2022 – 12/2/2022
Principles and techniques of Molecular Biology (MBT 505), 8/23/2021 – 12/3/2021
Colloquium Lecture Series (PSCI 425), 8/23/2021 – 12/3/2021
Colloquium Lecture Series (PSCI 425), 8/23/2021 – 12/3/2021
Spec Topics Biopharm Sci (BPS 594), 1/11/2021 – 4/30/2021
PDAT 9:Hematology/Oncology (Phar 509), 1/11/2021 – 5/7/2021
Molecular Biology, 8/24/2020 – 12/4/2020

Selected Grants

Genome Instability induced in cancer cells carrying mutations in Type II topoisomerases, National Institutes of Health (National Cancer Institute)., 12/20/2021 - 11/30/2023, Obligated Amount: $159900; Anticipated Amount: $159900

DNA Topoisomerase Misfunctioning in Neurological Disease, National Institutes of Health (National Institute of Neurological Disorders and Stroke)., 1/1/2021 - 11/30/2023, Obligated Amount: $159900; Anticipated Amount: $159900

Novel Approaches for Studying Topoisomerase 2 Targeting Anti-Cancer Drugs, National Institutes of Health (National Cancer Institute)., 4/1/2017 - 3/31/2020, Obligated Amount: $159900; Anticipated Amount: $159900

Regulation of Non-Homologous End Joining Through End Processing, National Institutes of Health (National Cancer Institute)., 7/1/2014 - 12/31/2017, Obligated Amount: $360967; Anticipated Amount: $360967

DNA Repair And Antitopoisomerase Drug Effects, National Institutes of Health (National Cancer Institute)., 9/2/2011 - 7/31/2012, Obligated Amount: $5953; Anticipated Amount: $5953

Anti-Topoisomerase Drug Action in Yeast, National Institutes of Health (National Cancer Institute)., 9/2/2011 - 1/31/2016, Obligated Amount: $982606; Anticipated Amount: $1008094

Studies of Childhood Solid Tumors, National Cancer Institute., 8/1/1978 - 7/31/2013, Obligated Amount: $43592000; No Anticipated Amount Set

Selected Publications

Sun, Y, Soans, E, Mishina, M, Petricci, E, Pommier, Y, Nitiss, KC, Nitiss, JL. (2022). Requirements for MRN endonuclease processing of topoisomerase II-mediated DNA damage in mammalian cells. Frontiers in Molecular Biosciences, 9. doi:10.3389/fmolb.2022.1007064.

Sun, Y, Nitiss, JL, Pommier, Y. (2022). SUMO: A Swiss Army Knife for Eukaryotic Topoisomerases. Frontiers in Molecular Biosciences, 9. doi:10.3389/fmolb.2022.871161.

Boot, A, Liu, M, Stantial, N, Shah, V, Yu, W, Nitiss, KC, Nitiss, JL, Jinks-Robertson, S, Rozen, SG. (2022). Recurrent mutations in topoisomerase IIα cause a previously undescribed mutator phenotype in human cancers. Proceedings of the National Academy of Sciences of the United States of America, 119, (4). doi:10.1073/pnas.2114024119.

Menendez, D, Anand, JR, Murphy, CC, Bell, WJ, Fu, J, Slepushkina, N, Buehler, E, Martin, SE, Lal-Nag, M, Nitiss, JL, Resnick, MA. (2022). Etoposide-induced DNA damage is increased in p53 mutants: identification of ATR and other genes that influence effects of p53 mutations on Top2-induced cytotoxicity. Oncotarget, 13, 332-346. doi:10.18632/ONCOTARGET.28195.

Nitiss, JL, Kiianitsa, K, Sun, Y, Nitiss, KC, Maizels, N. (2021). Topoisomerase Assays. Current Protocols, 1, (10). doi:10.1002/cpz1.250.

Sun, Y, Jenkins, LMM, Su, YP, Nitiss, KC, Nitiss, JL, Pommier, Y. (2020). A conserved SUMO pathway repairs topoisomerase DNA-protein cross-links by engaging ubiquitin-mediated proteasomal degradation. Science Advances, 6, (46). doi:10.1126/sciadv.aba6290.

Stantial, N, Rogojina, A, Gilbertson, M, Sun, Y, Miles, H, Shaltz, S, Berger, J, Nitiss, KC, Jinks-Robertson, S, Nitiss, JL. (2020). Trapped topoisomerase II initiates formation of de novo duplications via the nonhomologous end-joining pathway in yeast. Proceedings of the National Academy of Sciences of the United States of America, 117, (43), 26876-26884. doi:10.1073/pnas.2008721117.

Wang, Y, Rakela, S, Chambers, JW, Hua, ZC, Muller, MT, Nitiss, JL, Tse-Dinh, YC, Leng, F. (2019). Kinetic Study of DNA Topoisomerases by Supercoiling-Dependent Fluorescence Quenching. ACS Omega, 4, (19), 18413-18422. doi:10.1021/acsomega.9b02676.

Nitiss, KC, Nitiss, JL, Hanakahi, LA. (2019). DNA Damage by an essential enzyme: A delicate balance act on the tightrope. DNA Repair, 82. doi:10.1016/j.dnarep.2019.102639.

Education

Degrees:
Ph.D., Illinois Institute of Technology, United States, 1986
S.B., University of Chicago, United States, 1978

Postgraduate Training:
Post-doctoral fellow, Harvard University, United States, 1989