Photo of Dr. Alexander Mankin

Alexander Mankin


Professor, Center for Biomolecular Sciences
The Alexander Neyfakh Professor of Medicinal Chemistry and Pharmacognosy, Pharmaceutical Sciences

Contact

Building & Room:

3052 MBRB

Address:

900 S Ashland Avenue, Chicago, IL, 60607

Office Phone:

(312) 413-1406

Fax:

(312) 413-9303

Email:

shura@uic.edu

CV Link:

Alexander Mankin

Related Sites:

About

Research Interests: Mechanisms of protein synthesis; structure, function and evolution of ribosome and ribosomal RNA; mechanisms of action of ribosome-targeted antibiotics.
Teaching Interests: Fundamental principles of gene expression
Genomics
Mechanisms of antibiotic action
Antibiotic resistance

Selected Publications

  • Huang S, Aleksashin N, Loveland AB, Klepacki D, Reier K, Kefi A, Szal TM, Remme J, Jaeger L, Vázquez-Laslop N, Korostelev AA, Mankin A. Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly.. Nature communications. 2020;11(1):2900. doi:10.1038/s41467-020-16694-8.

  • Aleksashin N, Szal TM, d’Aquino AE, Jewett MC, Vázquez-Laslop N, Mankin A. A fully orthogonal system for protein synthesis in bacterial cells.. Nature communications. 2020;11(1):1858. doi:10.1038/s41467-020-15756-1.

  • Choi J, Marks JP, Zhang J, Chen DH, Wang J, Vázquez-Laslop N, Mankin A, Puglisi JD. Dynamics of the context-specific translation arrest by chloramphenicol and linezolid.. Nature chemical biology. 2020;16(3):310-317. doi:10.1038/s41589-019-0423-2.

  • Svetlov M, Cohen S, Alsuhebany N, Vázquez-Laslop N, Mankin A. A long-distance rRNA base pair impacts the ability of macrolide antibiotics to kill bacteria.. Proceedings of the National Academy of Sciences of the United States of America. 2020;117(4):1971-1975. doi:10.1073/pnas.1918948117.

  • Carlson ED, d’Aquino AE, Kim DS, Fulk EM, Hoang K, Szal TM, Mankin A, Jewett MC. Engineered ribosomes with tethered subunits for expanding biological function.. Nature communications. 2019;10(1):3920. doi:10.1038/s41467-019-11427-y.

  • Meydan S, Marks JP, Klepacki D, Sharma V, Baranov PV, Firth AE, Margus T, Kefi A, Vázquez-Laslop N, Mankin A. Retapamulin-Assisted Ribosome Profiling Reveals the Alternative Bacterial Proteome.. Molecular cell. 2019;74(3):481-493.e6. doi:10.1016/j.molcel.2019.02.017.

  • Aleksashin N, Leppik M, Hockenberry AJ, Klepacki D, Vázquez-Laslop N, Jewett MC, Remme J, Mankin A. Assembly and functionality of the ribosome with tethered subunits.. Nature communications. 2019;10(1):930. doi:10.1038/s41467-019-08892-w.

  • Vázquez-Laslop N, Mankin A. Context-Specific Action of Ribosomal Antibiotics.. Annual review of microbiology. 2018;72:185-207. doi:10.1146/annurev-micro-090817-062329.

  • Vázquez-Laslop N, Mankin A. How Macrolide Antibiotics Work.. Trends in biochemical sciences. 2018;43(9):668-684. doi:10.1016/j.tibs.2018.06.011.

  • Meydan S, Vázquez-Laslop N, Mankin A. Genes within Genes in Bacterial Genomes.. Microbiology spectrum. 2018;6(4). doi:10.1128/microbiolspec.RWR-0020-2018.

  • Pantel L, Florin T, Dobosz-Bartoszek M, Racine E, Sarciaux M, Serri M, Houard J, Campagne JM, de Figueiredo RM, Midrier C, Gaudriault S, Givaudan A, Lanois A, Forst S, Aumelas A, Cotteaux-Lautard C, Bolla JM, Vingsbo Lundberg C, Huseby DL, Hughes D, Villain-Guillot P, Mankin A, Polikanov YS, Gualtieri M. Odilorhabdins, Antibacterial Agents that Cause Miscoding by Binding at a New Ribosomal Site.. Molecular cell. 2018;70(1):83-94.e7. doi:10.1016/j.molcel.2018.03.001.

  • Tereshchenkov AG, Dobosz-Bartoszek M, Osterman IA, Marks JP, Sergeeva VA, Kasatsky P, Komarova ES, Stavrianidi AN, Rodin IA, Konevega AL, Sergiev PV, Sumbatyan NV, Mankin A, Bogdanov AA, Polikanov YS. Binding and Action of Amino Acid Analogs of Chloramphenicol upon the Bacterial Ribosome.. Journal of molecular biology. 2018;430(6):842-852. doi:10.1016/j.jmb.2018.01.016.

  • Svetlov M, Vázquez-Laslop N, Mankin A. Kinetics of drug-ribosome interactions defines the cidality of macrolide antibiotics.. Proceedings of the National Academy of Sciences of the United States of America. 2017;114(52):13673-13678. doi:10.1073/pnas.1717168115.

  • Almutairi M, Svetlov M, Hansen DA, Khabibullina NF, Klepacki D, Kang HY, Sherman DH, Vázquez-Laslop N, Polikanov YS, Mankin A. Co-produced natural ketolides methymycin and pikromycin inhibit bacterial growth by preventing synthesis of a limited number of proteins.. Nucleic acids research. 2017;45(16):9573-9582. doi:10.1093/nar/gkx673.

  • Florin T, Maracci C, Graf M, Karki P, Klepacki D, Berninghausen O, Beckmann R, Vázquez-Laslop N, Wilson DN, Rodnina MV, Mankin A. An antimicrobial peptide that inhibits translation by trapping release factors on the ribosome.. Nature structural & molecular biology. 2017;24(9):752-757. doi:10.1038/nsmb.3439.

  • Meydan S, Klepacki D, Karthikeyan S, Margus T, Thomas P, Jones JE, Khan Y, Briggs J, Dinman JD, Vázquez-Laslop N, Mankin A. Programmed Ribosomal Frameshifting Generates a Copper Transporter and a Copper Chaperone from the Same Gene.. Molecular cell. 2017;65(2):207-219. doi:10.1016/j.molcel.2016.12.008.

  • Marks JP, Kannan K, Roncase EJ, Klepacki D, Kefi A, Orelle C, Vázquez-Laslop N, Mankin A. Context-specific inhibition of translation by ribosomal antibiotics targeting the peptidyl transferase center.. Proceedings of the National Academy of Sciences of the United States of America. 2016;113(43):12150-12155. doi:10.1073/pnas.1613055113.

  • Sothiselvam S, Neuner S, Rigger L, Klepacki D, Micura R, Vázquez-Laslop N, Mankin A. Binding of Macrolide Antibiotics Leads to Ribosomal Selection against Specific Substrates Based on Their Charge and Size.. Cell reports. 2016;16(7):1789-99. doi:10.1016/j.celrep.2016.07.018.

  • Gagnon MG, Roy RN, Lomakin IB, Florin T, Mankin A, Steitz TA. Structures of proline-rich peptides bound to the ribosome reveal a common mechanism of protein synthesis inhibition.. Nucleic acids research. 2016;44(5):2439-50. doi:10.1093/nar/gkw018.

  • Gupta P, Liu B, Klepacki D, Gupta V, Schulten K, Mankin A, Vázquez-Laslop N. Nascent peptide assists the ribosome in recognizing chemically distinct small molecules.. Nature chemical biology. 2016;12(3):153-8. doi:10.1038/nchembio.1998.

  • Almutairi M, Park SR, Rose S, Hansen DA, Vázquez-Laslop N, Douthwaite S, Sherman DH, Mankin A. Resistance to ketolide antibiotics by coordinated expression of rRNA methyltransferases in a bacterial producer of natural ketolides.. Proceedings of the National Academy of Sciences of the United States of America. 2015;112(42):12956-61. doi:10.1073/pnas.1512090112.

  • Orelle C, Carlson ED, Szal TM, Florin T, Jewett MC, Mankin A. Protein synthesis by ribosomes with tethered subunits.. Nature. 2015;524(7563):119-24. doi:10.1038/nature14862.

  • Polikanov YS, Osterman IA, Szal TM, Tashlitsky VN, Serebryakova MV, Kusochek P, Bulkley D, Malanicheva IA, Efimenko TA, Efremenkova OV, Konevega AL, Shaw KJ, Bogdanov AA, Rodnina MV, Dontsova OA, Mankin A, Steitz TA, Sergiev PV. Amicoumacin a inhibits translation by stabilizing mRNA interaction with the ribosome.. Molecular cell. 2014;56(4):531-40. doi:10.1016/j.molcel.2014.09.020.

  • Polikanov YS, Szal TM, Jiang F, Gupta P, Matsuda R, Shiozuka M, Steitz TA, Vázquez-Laslop N, Mankin A. Negamycin interferes with decoding and translocation by simultaneous interaction with rRNA and tRNA.. Molecular cell. 2014;56(4):541-50. doi:10.1016/j.molcel.2014.09.021.

  • Kannan K, Kanabar P, Schryer D, Florin T, Oh E, Bahroos N, Tenson T, Weissman JS, Mankin A. The general mode of translation inhibition by macrolide antibiotics.. Proceedings of the National Academy of Sciences of the United States of America. 2014;111(45):15958-63. doi:10.1073/pnas.1417334111.

  • Sothiselvam S, Liu B, Han W, Ramu H, Klepacki D, Atkinson GC, Brauer A, Remm M, Tenson T, Schulten K, Vázquez-Laslop N, Mankin A. Macrolide antibiotics allosterically predispose the ribosome for translation arrest.. Proceedings of the National Academy of Sciences of the United States of America. 2014;111(27):9804-9. doi:10.1073/pnas.1403586111.

  • Vázquez-Laslop N, Mankin A. Protein accounting in the cellular economy.. Cell. 2014;157(3):529-31. doi:10.1016/j.cell.2014.04.002.

  • Arenz S, Ramu H, Gupta P, Berninghausen O, Beckmann R, Vázquez-Laslop N, Mankin A, Wilson DN. Molecular basis for erythromycin-dependent ribosome stalling during translation of the ErmBL leader peptide.. Nature communications. 2014;5:3501. doi:10.1038/ncomms4501.

  • Martínez AK, Gordon E, Sengupta A, Shirole N, Klepacki D, Martinez-Garriga B, Brown LM, Benedik MJ, Yanofsky C, Mankin A, Vazquez-Laslop NC, Sachs MS, Cruz-Vera LR. Interactions of the TnaC nascent peptide with rRNA in the exit tunnel enable the ribosome to respond to free tryptophan.. Nucleic acids research. 2014;42(2):1245-56. doi:10.1093/nar/gkt923.

  • Gupta P, Kannan K, Mankin A, Vázquez-Laslop N. Regulation of gene expression by macrolide-induced ribosomal frameshifting.. Molecular cell. 2013;52(5):629-42. doi:10.1016/j.molcel.2013.10.013.

  • Orelle C, Carlson SN, Kaushal B, Almutairi MM, Liu H, Ochabowicz A, Quan S, Pham VC, Squires CL, Murphy BT, Mankin A. Tools for characterizing bacterial protein synthesis inhibitors.. Antimicrobial agents and chemotherapy. 2013;57(12):5994-6004. doi:10.1128/AAC.01673-13.

  • Orelle C, Szal TM, Klepacki D, Shaw KJ, Vázquez-Laslop N, Mankin A. Identifying the targets of aminoacyl-tRNA synthetase inhibitors by primer extension inhibition.. Nucleic acids research. 2013;41(14):e144. doi:10.1093/nar/gkt526.

  • LaMarre J, Mendes RE, Szal TM, Schwarz S, Jones RN, Mankin A. The genetic environment of the cfr gene and the presence of other mechanisms account for the very high linezolid resistance of Staphylococcus epidermidis isolate 426-3147L.. Antimicrobial agents and chemotherapy. 2013;57(3):1173-9. doi:10.1128/AAC.02047-12.

  • Gupta P, Sothiselvam S, Vázquez-Laslop N, Mankin A. Deregulation of translation due to post-transcriptional modification of rRNA explains why erm genes are inducible.. Nature communications. 2013;4:1984. doi:10.1038/ncomms2984.

  • Kannan K, Vázquez-Laslop N, Mankin A. Selective protein synthesis by ribosomes with a drug-obstructed exit tunnel.. Cell. 2012;151(3):508-20. doi:10.1016/j.cell.2012.09.018.

  • Locke JB, Rahawi S, Lamarre J, Mankin A, Shaw KJ. Genetic environment and stability of cfr in methicillin-resistant Staphylococcus aureus CM05.. Antimicrobial agents and chemotherapy. 2012;56(1):332-40. doi:10.1128/AAC.05420-11.

  • Kannan K, Mankin A. Macrolide antibiotics in the ribosome exit tunnel: species-specific binding and action.. Annals of the New York Academy of Sciences. 2011;1241:33-47. doi:10.1111/j.1749-6632.2011.06315.x.

  • LaMarre JM, Locke JB, Shaw KJ, Mankin A. Low fitness cost of the multidrug resistance gene cfr.. Antimicrobial agents and chemotherapy. 2011;55(8):3714-9. doi:10.1128/AAC.00153-11.

  • Vázquez-Laslop N, Klepacki D, Mulhearn DC, Ramu H, Krasnykh O, Franzblau SG, Mankin A. Role of antibiotic ligand in nascent peptide-dependent ribosome stalling.. Proceedings of the National Academy of Sciences of the United States of America. 2011;108(26):10496-501. doi:10.1073/pnas.1103474108.

  • LaMarre JM, Howden BP, Mankin A. Inactivation of the indigenous methyltransferase RlmN in Staphylococcus aureus increases linezolid resistance.. Antimicrobial agents and chemotherapy. 2011;55(6):2989-91. doi:10.1128/AAC.00183-11.

  • Vázquez-Laslop N, Mankin A. Picky nascent peptides do not talk to foreign ribosomes.. Proceedings of the National Academy of Sciences of the United States of America. 2011;108(15):5931-2. doi:10.1073/pnas.1103011108.

  • Ramu H, Vázquez-Laslop N, Klepacki D, Dai Q, Piccirilli J, Micura R, Mankin A. Nascent peptide in the ribosome exit tunnel affects functional properties of the A-site of the peptidyl transferase center.. Molecular cell. 2011;41(3):321-30. doi:10.1016/j.molcel.2010.12.031.

  • Llano-Sotelo B, Dunkle J, Klepacki D, Zhang W, Fernandes P, Cate JH, Mankin A. Binding and action of CEM-101, a new fluoroketolide antibiotic that inhibits protein synthesis.. Antimicrobial agents and chemotherapy. 2010;54(12):4961-70. doi:10.1128/AAC.00860-10.

  • Dunkle JA, Xiong L, Mankin A, Cate JH. Structures of the Escherichia coli ribosome with antibiotics bound near the peptidyl transferase center explain spectra of drug action.. Proceedings of the National Academy of Sciences of the United States of America. 2010;107(40):17152-7. doi:10.1073/pnas.1007988107.

  • David-Eden H, Mankin A, Mandel-Gutfreund Y. Structural signatures of antibiotic binding sites on the ribosome.. Nucleic acids research. 2010;38(18):5982-94. doi:10.1093/nar/gkq411.

  • Vázquez-Laslop N, Ramu H, Klepacki D, Kannan K, Mankin A. The key function of a conserved and modified rRNA residue in the ribosomal response to the nascent peptide.. The EMBO journal. 2010;29(18):3108-17. doi:10.1038/emboj.2010.180.

  • Yan F, LaMarre JM, Röhrich R, Wiesner J, Jomaa H, Mankin A, Fujimori DG. RlmN and Cfr are radical SAM enzymes involved in methylation of ribosomal RNA.. Journal of the American Chemical Society. 2010;132(11):3953-64. doi:10.1021/ja910850y.

  • Auerbach T, Mermershtain I, Davidovich C, Bashan A, Belousoff M, Wekselman I, Zimmerman E, Xiong L, Klepacki D, Arakawa K, Kinashi H, Mankin A, Yonath A. The structure of ribosome-lankacidin complex reveals ribosomal sites for synergistic antibiotics.. Proceedings of the National Academy of Sciences of the United States of America. 2010;107(5):1983-8. doi:10.1073/pnas.0914100107.

  • Llano-Sotelo B, Klepacki D, Mankin A. Selection of small peptides, inhibitors of translation.. Journal of molecular biology. 2009;391(5):813-9. doi:10.1016/j.jmb.2009.06.069.

  • Llano-Sotelo B, Hickerson RP, Lancaster L, Noller HF, Mankin A. Fluorescently labeled ribosomes as a tool for analyzing antibiotic binding.. RNA (New York, N.Y.). 2009;15(8):1597-604. doi:10.1261/rna.1681609.

  • Mankin A. Macrolide myths.. Current opinion in microbiology. 2008;11(5):414-21. doi:10.1016/j.mib.2008.08.003.

  • Toh SM, Mankin A. An indigenous posttranscriptional modification in the ribosomal peptidyl transferase center confers resistance to an array of protein synthesis inhibitors.. Journal of molecular biology. 2008;380(4):593-7. doi:10.1016/j.jmb.2008.05.027.

  • , Mankin A. Transcriptional and translational control of the mlr operon, which confers resistance to seven classes of protein synthesis inhibitors.. Antimicrobial agents and chemotherapy. 2008;52(5):1703-12. doi:10.1128/AAC.01583-07.

  • Vazquez-Laslop NC, Thum C, Mankin A. Molecular mechanism of drug-dependent ribosome stalling.. Molecular cell. 2008;30(2):190-202. doi:10.1016/j.molcel.2008.02.026.

  • , Chettiath T, Mankin A. Induction of erm(C) expression by noninducing antibiotics.. Antimicrobial agents and chemotherapy. 2008;52(3):866-74. doi:10.1128/AAC.01266-07.

  • Samant S, Lee H, Ghassemi M, Chen J, Cook JL, Mankin A, Neyfakh AA. Nucleotide biosynthesis is critical for growth of bacteria in human blood.. PLoS pathogens. 2008;4(2):e37. doi:10.1371/journal.ppat.0040037.

  • Mankin A, Polacek N. SPARK: a new peptidyl transferase activity assay.. Methods in molecular medicine. 2008;142:107-16. doi:10.1007/978-1-59745-246-5_9.

  • Toh SM, Xiong L, Bae T, Mankin A. The methyltransferase YfgB/RlmN is responsible for modification of adenosine 2503 in 23S rRNA.. RNA (New York, N.Y.). 2008;14(1):98-106. doi:10.1261/rna.814408.

  • Yassin A, Mankin A. Potential new antibiotic sites in the ribosome revealed by deleterious mutations in RNA of the large ribosomal subunit.. The Journal of biological chemistry. 2007;282(33):24329-42. doi:10.1074/jbc.M703106200.

  • Toh SM, Xiong L, Arias CA, Villegas MV, Lolans K, Quinn J, Mankin A. Acquisition of a natural resistance gene renders a clinical strain of methicillin-resistant Staphylococcus aureus resistant to the synthetic antibiotic linezolid.. Molecular microbiology. 2007;64(6):1506-14. doi:10.1111/j.1365-2958.2007.05744.x.

  • Leach KL, Swaney SM, Colca JR, McDonald WG, Blinn JR, Thomasco LM, Gadwood RC, Shinabarger D, Xiong L, Mankin A. The site of action of oxazolidinone antibiotics in living bacteria and in human mitochondria.. Molecular cell. 2007;26(3):393-402. doi:10.1016/j.molcel.2007.04.005.

  • Sherman D, Xiong L, Mankin A, Melman A. Synthesis and biological investigation of new 4”-malonyl tethered derivatives of erythromycin and clarithromycin.. Bioorganic & medicinal chemistry letters. 2006;16(6):1506-9. doi:10.1016/j.bmcl.2005.12.033.

  • Mankin A. Nascent peptide in the “birth canal” of the ribosome.. Trends in biochemical sciences. 2006;31(1):11-3. doi:10.1016/j.tibs.2005.11.007.

  • Yassin A, Fredrick K, Mankin A. Deleterious mutations in small subunit ribosomal RNA identify functional sites and potential targets for antibiotics.. Proceedings of the National Academy of Sciences of the United States of America. 2005;102(46):16620-5. doi:10.1073/pnas.0508444102.

  • Maguire BA, Beniaminov AD, Ramu H, Mankin A, Zimmermann RA. A protein component at the heart of an RNA machine: the importance of protein l27 for the function of the bacterial ribosome.. Molecular cell. 2005;20(3):427-35. doi:10.1016/j.molcel.2005.09.009.

  • Beringer M, Bruell C, Xiong L, Pfister P, Bieling P, Katunin VI, Mankin A, Böttger EC, Rodnina MV. Essential mechanisms in the catalysis of peptide bond formation on the ribosome.. The Journal of biological chemistry. 2005;280(43):36065-72. doi:10.1074/jbc.M507961200.

  • Polacek N, Mankin A. The ribosomal peptidyl transferase center: structure, function, evolution, inhibition.. Critical reviews in biochemistry and molecular biology. 2005;40(5):285-311. doi:10.1080/10409230500326334.

  • Erlacher MD, Lang K, Shankaran N, Wotzel B, Hüttenhofer A, Micura R, Mankin A, Polacek N. Chemical engineering of the peptidyl transferase center reveals an important role of the 2′-hydroxyl group of A2451.. Nucleic acids research. 2005;33(5):1618-27. doi:10.1093/nar/gki308.

  • Xiong L, Korkhin Y, Mankin A. Binding site of the bridged macrolides in the Escherichia coli ribosome.. Antimicrobial agents and chemotherapy. 2005;49(1):281-8. doi:10.1128/AAC.49.1.281-288.2005.

  • Ruggero KA, Schroeder LK, Schreckenberger PC, Mankin A, Quinn JP. Nosocomial superinfections due to linezolid-resistant Enterococcus faecalis: evidence for a gene dosage effect on linezolid MICs.. Diagnostic microbiology and infectious disease. 2003;47(3):511-3.

  • Colca JR, McDonald WG, Waldon DJ, Thomasco LM, Gadwood RC, Lund ET, Cavey GS, Mathews WR, Adams LD, Cecil ET, Pearson JD, Bock JH, Mott JE, Shinabarger DL, Xiong L, Mankin A. Cross-linking in the living cell locates the site of action of oxazolidinone antibiotics.. The Journal of biological chemistry. 2003;278(24):21972-9. doi:10.1074/jbc.M302109200.

  • Gaynor M, Mankin A. Macrolide antibiotics: binding site, mechanism of action, resistance.. Current topics in medicinal chemistry. 2003;3(9):949-61.

  • Sander P, Belova L, Kidan YG, Pfister P, Mankin A, Böttger EC. Ribosomal and non-ribosomal resistance to oxazolidinones: species-specific idiosyncrasy of ribosomal alterations.. Molecular microbiology. 2002;46(5):1295-304.

  • Polacek N, Swaney S, Shinabarger D, Mankin A. SPARK–a novel method to monitor ribosomal peptidyl transferase activity.. Biochemistry. 2002;41(39):11602-10.

  • Tenson T, Mankin A. Short peptides conferring resistance to macrolide antibiotics.. Peptides. 2001;22(10):1661-8.

  • Mann PA, Xiong L, Mankin A, Chau AS, Mendrick CA, Najarian DJ, Cramer CA, Loebenberg D, Coates E, Murgolo NJ, Aarestrup FM, Goering RV, Black TA, Hare RS, McNicholas PM. EmtA, a rRNA methyltransferase conferring high-level evernimicin resistance.. Molecular microbiology. 2001;41(6):1349-56.

  • Polacek N, Gaynor M, Yassin A, Mankin A. Ribosomal peptidyl transferase can withstand mutations at the putative catalytic nucleotide.. Nature. 2001;411(6836):498-501. doi:10.1038/35078113.

  • Belova L, Tenson T, Xiong L, McNicholas PM, Mankin A. A novel site of antibiotic action in the ribosome: interaction of evernimicin with the large ribosomal subunit.. Proceedings of the National Academy of Sciences of the United States of America. 2001;98(7):3726-31. doi:10.1073/pnas.071527498.

  • Bocchetta M, Xiong L, Shah S, Mankin A. Interactions between 23S rRNA and tRNA in the ribosomal E site.. RNA (New York, N.Y.). 2001;7(1):54-63.

  • Xiong L, Kloss P, Douthwaite S, Andersen NM, Swaney S, Shinabarger DL, Mankin A. Oxazolidinone resistance mutations in 23S rRNA of Escherichia coli reveal the central region of domain V as the primary site of drug action.. Journal of bacteriology. 2000;182(19):5325-31.

  • Kloss P, Xiong L, Shinabarger DL, Mankin A. Resistance mutations in 23 S rRNA identify the site of action of the protein synthesis inhibitor linezolid in the ribosomal peptidyl transferase center.. Journal of molecular biology. 1999;294(1):93-101. doi:10.1006/jmbi.1999.3247.

  • Khaitovich P, Mankin A. Effect of antibiotics on large ribosomal subunit assembly reveals possible function of 5 S rRNA.. Journal of molecular biology. 1999;291(5):1025-34. doi:10.1006/jmbi.1999.3030.

  • Mankin A, Liebman SW. (1999). Baby, don’t stop!. Nature genetics. Vol. 23. Issue 1. Page(s) 8-10.

  • Chernyaeva NS, Murgola EJ, Mankin A. Suppression of nonsense mutations induced by expression of an RNA complementary to a conserved segment of 23S rRNA.. Journal of bacteriology. 1999;181(17):5257-62.

  • Khaitovich P, Tenson T, Mankin A, Green R. (1999). Peptidyl transferase activity catalyzed by protein-free 23S ribosomal RNA remains elusive.. RNA (New York, N.Y.). Vol. 5. Issue 5. Page(s) 605-8.

  • Tenson T, Herrera JV, Kloss P, Guarneros G, Mankin A. Inhibition of translation and cell growth by minigene expression.. Journal of bacteriology. 1999;181(5):1617-22.

  • Khaitovich P, Tenson T, Kloss P, Mankin A. Reconstitution of functionally active Thermus aquaticus large ribosomal subunits with in vitro-transcribed rRNA.. Biochemistry. 1999;38(6):1780-8. doi:10.1021/bi9822473.

  • Khaitovich P, Mankin A, Green R, Lancaster L, Noller HF. Characterization of functionally active subribosomal particles from Thermus aquaticus.. Proceedings of the National Academy of Sciences of the United States of America. 1999;96(1):85-90.

  • Xiong L, Shah S, Mauvais P, Mankin A. A ketolide resistance mutation in domain II of 23S rRNA reveals the proximity of hairpin 35 to the peptidyl transferase centre.. Molecular microbiology. 1999;31(2):633-9.

  • Tripathi S, Kloss PS, Mankin A. Ketolide resistance conferred by short peptides.. The Journal of biological chemistry. 1998;273(32):20073-7.

  • Bocchetta M, Xiong L, Mankin A. 23S rRNA positions essential for tRNA binding in ribosomal functional sites.. Proceedings of the National Academy of Sciences of the United States of America. 1998;95(7):3525-30.

  • Porse BT, Leviev I, Mankin A, Garrett RA. The antibiotic thiostrepton inhibits a functional transition within protein L11 at the ribosomal GTPase centre.. Journal of molecular biology. 1998;276(2):391-404. doi:10.1006/jmbi.1997.1541.

  • Mankin A. Pactamycin resistance mutations in functional sites of 16 S rRNA.. Journal of molecular biology. 1997;274(1):8-15. doi:10.1006/jmbi.1997.1387.

  • Tenson T, Xiong L, Kloss P, Mankin A. Erythromycin resistance peptides selected from random peptide libraries.. The Journal of biological chemistry. 1997;272(28):17425-30.

  • Tan GT, DeBlasio A, Mankin A. Mutations in the peptidyl transferase center of 23 S rRNA reveal the site of action of sparsomycin, a universal inhibitor of translation.. Journal of molecular biology. 1996;261(2):222-30. doi:10.1006/jmbi.1996.0454.

  • Dam M, Douthwaite S, Tenson T, Mankin A. Mutations in domain II of 23 S rRNA facilitate translation of a 23 S rRNA-encoded pentapeptide conferring erythromycin resistance.. Journal of molecular biology. 1996;259(1):1-6. doi:10.1006/jmbi.1996.0296.

  • Mankin A, Leviev I, Garrett RA. Cross-hypersensitivity effects of mutations in 23 S rRNA yield insight into aminoacyl-tRNA binding.. Journal of molecular biology. 1994;244(2):151-7. doi:10.1006/jmbi.1994.1715.

  • Leviev IG, Rodriguez-Fonseca C, Phan H, Garrett RA, Heilek G, Noller HF, Mankin A. A conserved secondary structural motif in 23S rRNA defines the site of interaction of amicetin, a universal inhibitor of peptide bond formation.. The EMBO journal. 1994;13(7):1682-6.

  • Akhmanova AS, Kagramanova VK, Mankin A. Heterogeneity of small plasmids from halophilic archaea.. Journal of bacteriology. 1993;175(4):1081-6.

  • Mankin A, Zyrianova IM, Kagramanova VK, Garrett RA. Introducing mutations into the single-copy chromosomal 23S rRNA gene of the archaeon Halobacterium halobium by using an rRNA operon-based transformation system.. Proceedings of the National Academy of Sciences of the United States of America. 1992;89(14):6535-9.

  • Mankin A, Garrett RA. Chloramphenicol resistance mutations in the single 23S rRNA gene of the archaeon Halobacterium halobium.. Journal of bacteriology. 1991;173(11):3559-63.

  • Garrett RA, Dalgaard J, Larsen N, Kjems J, Mankin A. Archaeal rRNA operons.. Trends in biochemical sciences. 1991;16(1):22-6.

  • Mankin A. The nucleotide sequence of the genes coding for the S19 and L22 equivalent ribosomal proteins from Halobacterium halobium.. FEBS letters. 1989;246(1-2):13-6.

  • Spiridonova VA, Akhmanova AS, Kagramanova VK, Köpke AK, Mankin A. Ribosomal protein gene cluster of Halobacterium halobium: nucleotide sequence of the genes coding for S3 and L29 equivalent ribosomal proteins.. Canadian journal of microbiology. 1989;35(1):153-9.

  • Kagramanova VK, Derckacheva NI, Mankin A. Unusual nucleotide sequence heterogeneity of small multicopy pHSB plasmid from Halobacterium strain SB3, an archaebacterium.. Canadian journal of microbiology. 1989;35(1):160-3.

  • Mankin A, Kagramanova VK. Complex promoter pattern of the single ribosomal RNA operon of an archaebacterium Halobacterium halobium.. Nucleic acids research. 1988;16(10):4679-92.

  • Kagramanova VK, Derckacheva NI, Mankin A. The complete nucleotide sequence of the arcaebacterial plasmid pHSB from Halobacterium, strain SB3.. Nucleic acids research. 1988;16(9):4158.

  • Bogdanov AA, Chichkova NV, Kopylov AM, Mankin A, Skripkin EA. Surface topography of ribosomal RNA.. Methods in enzymology. 1988;164:440-56.

  • Mankin A, Skripkin EA, Kagramanova VK. A putative internal promoter in the 16 S/23 S intergenic spacer of the rRNA operon of archaebacteria and eubacteria.. FEBS letters. 1987;219(2):269-73.

  • Mankin A, Skryabin KG, Rubtsov PM. Identification of ten additional nucleotides in the primary structure of yeast 18S rRNA.. Gene. 1986;44(1):143-5.

  • Zueva VS, Mankin A, Bogdanov AA, Baratova LA. Specific fragmentation of tRNA and rRNA at a 7-methylguanine residue in the presence of methylated carrier RNA.. European journal of biochemistry / FEBS. 1985;146(3):679-87.

  • Mankin A, Kagramanova VK, Teterina NL, Rubtsov PM, Belova EN, Kopylov AM, Baratova LA, Bogdanov AA. The nucleotide sequence of the gene coding for the 16S rRNA from the archaebacterium Halobacterium halobium.. Gene. 1985;37(1-3):181-9.

  • Mankin A, Teterina NL, Rubtsov PM, Baratova LA, Kagramanova VK. Putative promoter region of rRNA operon from archaebacterium Halobacterium halobium.. Nucleic acids research. 1984;12(16):6537-46.

  • Kozlov YuV , Rupasov VV, Adyshev DM, Belgelarskaya SN, Agranovsky AA, Mankin A, Morozov SYu , Dolja VV, Atabekov JG. Nucleotide sequence of the 3′-terminal tRNA-like structure in barley stripe mosaic virus genome.. Nucleic acids research. 1984;12(9):4001-9.

  • Tyulkina LG, Mankin A. Inhibition of ribonuclease contamination in preparations of T4 RNA ligase, polynucleotide kinase, and bacterial alkaline phosphatase with bentonite.. Analytical biochemistry. 1984;138(2):285-90.

  • Vartapetian AB, Mankin A, Skripkin EA, Chumakov KM, Smirnov VD, Bogdanov AA. The primary and secondary structure of the 5′-end region of encephalomyocarditis virus RNA. A novel approach to sequencing long RNA molecules.. Gene. 1983;26(2-3):189-95.

  • Kagramanova VK, Mankin A, Baratova LA, Bogdanov AA. The 3′-terminal nucleotide sequence of the Halobacterium halobium 16 S rRNA.. FEBS letters. 1982;144(1):177-80.

  • Mankin A, Kopylov AM, Bogdanov AA. Modification of 18 S rRNA in the 40 S ribosomal subunit of yeast with dimethyl sulfate.. FEBS letters. 1981;134(1):11-4.

  • Mankin A, Skripkin EA, Chichkova NV, Kopylov AM, Bogdanov AA. An enzymatic approach for localization of oligodeoxyribonucleotide binding sites on RNA. Application to studying rRNA topography.. FEBS letters. 1981;131(2):253-6.

    • Notable Honors

    2014, Alexander A. Neyfakh Collegiate Professorship, UIC

    2014, American Academy of Microbiology, Fellow, American Academy of Microbiology

    2014, Distinguished UIC Professorship, UIC

    2013, Paul R. Dawson Biotechnology Award, American Association of the Colleges of Pharmacy

    2011, Outstanding Service Recognition, American Society for Microbiology

    2008, The UIC Researcher of the Year Award, UIC

    2006, Interscience Conference on Antimicrobial Agents and Chemotherapy (ICAAC)award for the best presented work,

    2005, Excellence in Teaching Award,

    2005, The University Scholar Award,

    2001, Teacher Recognition Program Award, UIC Center for Excellence in Teaching and Learning

    1999, Teacher of the year award,

    1998, Teacher of the year award,

    Education

  • D.Sci, D.Sci, Moscow University

  • PhD, Moscow University

  • MS, Moscow University